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we demonstrate that AidH functions

The researchers concluded: "The genus Marinobacter seemed to play a major role in the degradation of a variety of hydrocarbons, as discount tiffany rings members of this group were isolated from the different enrichment cultures and grew on plates with hexadecane or single PAHs as sole carbon sources.""In this study, we report the characterization of a novel AHL-degrading protein from the discount Tiffany Rings bacterium Ochrobactrum sp. strain T63. This protein, termed AidH, shares no similarity with any of the known AHL degradases discount tiffany rings is highly homologous with a hydrolytic enzyme from Ochrobactrum anthropi ATCC 49188 that contains the alpha/beta-hydrolase fold. By liquid chromatography-mass spectrometry (MS) analysis, we demonstrate that AidH functions as an AHL-lactonase that hydrolyzes the ester bond of the homoserine lactone ring of AHLs. Mutational discount tiffany rings indicate that the G-X-Nuc-X-G motif or the histidine residue conserved among alpha/beta-hydrolases is critical for the activity of AidH. Furthermore, the AHL-inactivating discount tiffany rings of AidH requires Mn(2+) but not several other tested divalent cations," wrote G.Y. Mei and colleagues, China Agricultural University, Department of Plant Pathology.